The different stages of micropropagation include:
Micropropagation is the process of multiplying plant stock plant material by growing plantlets in tissue culture to produce a large number of progeny plants and then planting them out.
The different stages of micropropagation include:
Selection of mother plant
Establishment of aseptic culture
Micropropagation is the production of many plants from a small plant part, whereas tissue culture is that step of micropropagation where plant cells are placed in an artificial medium and grown into a large number.
The bleach solution is added to a flask containing the explant and swirled. Rinse the bleach and wash the seeds with pure water.
Micropropagation helps in the propagation of a large number of plants in a very short time. The plantlets produced are healthy and with desired characteristics.
Micropropagation is the artificial process of producing plants vegetatively through tissue culture or cell culture techniques. In this artificial process of propagation, plants are produced invitro by asexual means of reproduction or by vegetative propagation.
Plants can be produced both asexually i.e, via vegetative parts’ multiplication or sexually i.e., seed production. One of the means of asexual reproduction is by multiplying genetic replicas of plants that are referred to as clonal propagation wherein plants can be populated from a single individual through asexual means of reproduction.
For the in vivo propagation of specific plants, asexual reproduction via multiplication of vegetative parts is the only resort since they do not generate functional seeds as seen in figs, grapes, bananas etc. Successful application of clonal propagation to the following is observed: potato, apple and many other ornamental plants.
These artificial process of producing plantlet involves 5 different methods:
In this method of micropropagation, subtending leaf primordial and a meristem is placed into their respective growing media culture and allowed to grow. After some weeks, an elongated rooted plantlet is produced. Once after they reach a considerable height, these plantlets are transferred into the soil. In this method, a disease-free plant can be produced and can be successfully used for rapid multiplication of various herbaceous plants.
In this method, selected plant tissue is placed in an artificial growing medium culture until the callus is formed. After the production of callus, they are transferred into a culture medium containing plant growth regulators for the induction of adventitious organs. After a few weeks, a new plantlet is exposed gradually to the environmental condition.
In this method of micropropagation, cells or groups of cells are dispersed and allowed to grow in an aerated and sterile liquid culture medium.
In the method of embryo culture, the embryo is extracted and placed into a culture medium with proper nutrient in aseptic condition.
In this method, the plant cell is isolated and cultured in an appropriate medium to reform the cell wall and callus. Later, under suitable conditions, the cell develops a cell wall followed by an increase in cell division and cellular differentiation and grows into a new plant.
This is the initial stage of micropropagation. The stock plants are selected and grown under controlled conditions before using them for culture initiation.
The explants are established in a suitable culture medium. This stage involves the following steps:
The explant is established on an appropriate culture medium.
This stage involves the rapid multiplication of shoots or rapid somatic embryo formation in a defined culture medium.
In this stage, the shoots are transferred to a medium for the development of roots. The shoots are either transferred directly in the soil for the root development or transferred to a nutrient medium in the laboratory.
In this stage, the plantlets are established in the soil. The shoots from the laboratory are transferred to a greenhouse under controlled conditions of temperature, humidity and light.
The micropropagation technique has proved beneficial in many ways. Following are the advantages of micropropagation in plant production:
This is an alternative method for vegetative propagation with enhanced multiplication rate.
Large quantities of identical plants can be obtained from a single plant tissue within a very short time period.
The shoot multiplication has a very short cycle and each cycle results in a logarithmic increase in the number of shoots.
The small-sized propagules can be stored and transported easily.
The germplasm stocks can be maintained for several years using this technique.
It helps in the production and maintenance of pathogen-free plant varieties.
In a dioecious plant, the seed progeny yield is 50% male and 50% female. This method helps in obtaining the desired sex of the plant.
Millions of plantlets can be maintained in the cultural vials.
Genetic uniformity of the propagules can be maintained through this technique.
New varieties of species can be propagated.
A requirement of less space and human resources.
This method is independent of season and can be carried out anytime.
Assists in the regenerating genetically modified cells after protoplast fusion.
Often produces healthier plants, leading to quicker growth compared to those plants produced by a conventional method.
The disadvantages of micropropagation are given below:
The plants produced are not autotrophic.
The plants find a problem acclimatizing to the natural environment.
Culture initiation and establishment
Transfer of plantlets in the greenhouse environment