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Introducing the desired gene into the genome of a host organism is a challenging process.

Introducing the desired gene into the genome of a host organism is a challenging process. It involves insertion of the selected gene into the host which is facilitated by a vector which helps integration of the gene to form the recombinant DNA. This DNA is then introduced inside the host, sustained and carried forward.

Cloning vector refers to a minute DNA molecule which self-replicates itself inside the host cell. Cloning vectors are utilized for duplicating the desired DNA fragment into the host cell. Features of a cloning vectors are:

Recombinant DNA technology changes the phenotype of an organism (host) with the help of a genetically transformed vector. The cloning vector is then inserted into the genome of the organism. The process involves the insertion of a desirable foreign DNA with the gene of interest into the genome of the host. This gene is called recombinant gene and this method or technique is called recombinant DNA technology. A recombinant DNA technology can be carried out and accomplished with the support of some fundamental tools. The different tools used for the function are mentioned below: Tools of Recombinant DNA technology

The integration of the gene of interest is done with the help of vectors. These are the vehicles that drive the gene inside the host organism. The most commonly used vectors are bacteriophages and plasmids for their high copy number.

The organism in which the recombinant DNA is introduced is called the host organism. It is the ultimate tool into which the vector drives the gene of interest using the enzymes. Techniques like microinjection, gene gun, biolistic are employed to insert the recombinant DNA within the organism. This can be also carried out using alternate heating and cooling or use of calcium ions. (image will be uploaded soon)

Introducing the desired gene into the genome of a host organism is a challenging process. It involves insertion of the selected gene into the host which is facilitated by a vector which helps integration of the gene to form the recombinant DNA. This DNA is then introduced inside the host, sustained and carried forward. Steps of recombinant DNA technology:

Recombinant DNA technology changes the phenotype of an organism (host) with the help of a genetically transformed vector. The cloning vector is then inserted into the genome of the organism. The process involves the insertion of a desirable foreign DNA with the gene of interest into the genome of the host. This gene is called recombinant gene and this method or technique is called recombinant DNA technology. A recombinant DNA technology can be carried out and accomplished with the support of some fundamental tools. Introducing the desired gene into the genome of a host organism is a challenging process. It involves insertion of the selected gene into the host which is facilitated by a vector which helps integration of the gene to form the recombinant DNA. This DNA is then introduced inside the host, sustained and carried forward.