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A vector may be a DNA molecule that won’t carry a far off DNA into the host cell.

A cloning vector may be a small piece of DNA into which a far off DNA is often inserted for cloning purposes. A vector may be a DNA molecule that won’t carry a far off DNA into the host cell. It has the ability to self replicate and integrate into the host cell. These vectors have played a major role in analyzing the molecular structure of DNA. Vectors are often a plasmid from the bacterium, a cell from the upper organism or DNA from an epidemic. The target DNA is inserted into the precise sites of the vector and ligated by DNA ligase. The vector is then changed into the host cell for replacement.

The cloning vectors possess the following features:

There are subsequent different types of cloning vectors:

For a proper genomic DNA cloning, the DNA to be cloned is extracted from the organism of interest. Virtually any tissue source is often used (even tissues from extinct animals) as long as because the DNA is not extensively degraded. The DNA is then purified using simple methods to remove contaminating proteins (extraction with phenol), RNA (ribonuclease), and smaller molecules (precipitation and/or chromatography). Polymerase chain reaction (PCR) methods are often used for amplification of specific DNA or RNA (by a process known as Reverse-Transcription or RT-PCR) sequences prior to molecular cloning using primers or short DNA sequences specific for the region of interest. DNA for cloning experiments can also be obtained from RNA using polymerase(complementary DNA or cDNA cloning), or within the sort of synthetic DNA (artificial gene synthesis). cDNA cloning is usually utilized to obtain clones representative of the mRNA population of the cells of interest, while synthetic DNA is employed to get any precise sequence defined by the designer.

Cloning vectors are small pieces to insert foreign DNA into another cell and make multiple copies for an equivalent. The foreign DNA is replaced and expressed using the host cell machinery. It amplifies one copy of DNA into multiple copies.  

Q1. What is Cloning Vector? Ans – A cloning vector may be a small piece of DNA into which a far off DNA is often inserted for cloning purposes. A vector may be a DNA molecule that won’t carry a far off DNA into the host cell. It has the ability to self replicate and integrate into the host cell. These vectors have played a major role in analyzing the molecular structure of DNA. Vectors are often a plasmid from the bacterium, a cell from the upper organism or DNA from an epidemic. The target DNA is inserted into the precise sites of the vector and ligated by DNA ligase. The vector is then changed into the host cell for replacement. Q2. What are the types of Cloning Vector? Ans – The types of Cloning vector are –

Q3. What are Plasmids? Ans – Plasmids are the primary vectors utilized in gene cloning. These are found in bacteria, eukaryotes, and archaea. These are natural, extrachromosomal, self-replicating DNA molecules. They possess antibiotic-resistant genes along with high copy numbers. They encode proteins that are necessary for their own replication. pBR322, pUC18, F-plasmid are some of the examples of plasmid vectors.